Custom Rabbit Polyclonal
|
Custom Mouse Polyclonal
|
Custom Rat Polyclonal
|
Steps | Time from Project Initiation | Time from Immunization | Activity Description |
---|---|---|---|
Peptide synthesis | ≈2 weeks | Day 21 | - |
Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
Primary immunization | ≈4 weeks | Day 0 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Complete Adjuvant, Subcutaneous administration |
1st boost | ≈6 weeks | Day 14 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
2nd boost | ≈8 weeks | Day 28 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
Production bleed | ≈9 weeks | Day 35 | 10-20 ml serum per rabbit |
3rd boost (SC1044 only) |
≈10 weeks | Day 42 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
Production bleed | ≈11 weeks | Day 49 | 40 ml serum per rabbit |
ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |
Steps | Time from Project Initiation | Time from Immunization | Activity Description |
---|---|---|---|
Peptide synthesis | ≈2 weeks | Day 21 | - |
Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
Primary immunization | ≈4 weeks | Day 0 | 50 μg per mouse peptide-KLH conjugate, Freud's Complete Adjuvant, Intrapreitoneal injection |
1st boost | ≈6 weeks | Day 14 | 25 μg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection |
2nd boost | ≈8 weeks | Day 28 | 25 μg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection |
Test bleed and ELISA | ≈9 weeks | Day 35 | 0.01-0.02 ml serum per mouse. ELISA and response evaluation |
3rd boost | ≈10 weeks | Day 42 | 25 μg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection |
Production bleed | ≈11 weeks | Day 49 | 0.5 ml serum per mouse. |
ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |
Steps | Time from Project Initiation | Time from Immunization | Activity Description |
---|---|---|---|
Peptide synthesis | ≈2 weeks | Day 21 | - |
Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
Primary immunization | ≈4 weeks | Day 0 | 50 μg per rat peptide-KLH conjugate, -Freunds's Complete Adjuvant, Intraperitoneal injection |
1st boost | ≈6 weeks | Day 14 | 25 μg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
2nd boost | ≈8 weeks | Day 28 | 25 μg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
Test bleed and ELISA | ≈9 weeks | Day 35 | 0.01-0.02 ml serum per rat. ELISA and initial response evaluation |
3rd boost | ≈10 weeks | Day 42 | 25 μg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
Production bleed | ≈11 weeks | Day 49 | 1.5-2 ml serum per rat |
ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |